The cheapest way to immobilize enzyme having following property
1. should be in powder form
2. The powder should be soluble at pH 6-7
3. should be eatable.
4. activity loss should be minimum.
I think it will be very cheap with entrapment or encapsulation technology using alginate nanoparticle or alginate microsphere/microcapsule. It would be:
1. In powder form
2. Not soluble, but form suspension in water.
3. Should be edible, since alginate is biodegradable.
4. Many study shows entrapment/encapsulation with polysaccharide-based nanoparticles could prevent activity loss.
Reference:
http://scholar.google.com/scholar?cluster=15018389258631515498&hl=en&as_sdt=0,5
http://scholar.google.com/scholar?cluster=1387134144772405987&hl=en&as_sdt=0,5
Hope that help,
Radif
Thank you Muhammad Radifar but alginate not working for me as alginate need crosslinker that could be ionic like Ca++ etc I am working on emulsion and enzyme is in crude form. Can you tell me can I use Spray drier for it.
What is the ultimate purpose of this enzyme preparation? Because if your enzyme is naked it could be digested by proteases inside the digestive tract, unless if your enzyme is digestive tract enzyme which purpose is to aid the food digestion, which mean your enzyme should be naked.
And why would Ca2+ is dangerous? As far as I can tell the use of alginate & Ca2+ in the above paper is to minimize the use of reactive chemical that could damage/denature the protein. Is it because the enzyme is inside emulsion and the emulsion would break with the addition of ion molecule?
What about SEDDS (Self-Emulsifying Drug Delivery System), here is a review that discuss many SEDDS-based protein/peptide delivery:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2676808/
PS: I'm not familiar with spray dryer, sorry.
I recommend the CLEAs, cross-linked enzyme aggregates. It is a self-containing enzyme immobilization with no need for support. One reference from the creator of CLEAs: http://dx.doi.org/10.1016/S0958-1669(03)00096-X
Why immobilize if you need soluble enzyme?
Maybe CLEA can do the work. However, if you need it soluble don't immobilize them.
Aura tagliani, André Soibelmann Glock Lorenzoni, Pedro Luis Valencia thank you for your answer. I am working on Arg protease that is partialy purified and I will be using it during emulsion process of natural material to developed a functional food for oral consumption. For cheap and easy packaging i want that crude to be converted to powder form with high stability.
Accidentally found this, I think it suit all the requirement you mentioned above
http://www.nature.com/nbt/journal/v10/n9/abs/nbt0992-1007.html
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