Does anybody know a supplier for tmTCEP (trimethyl TCEP)?

I found different publications about methylated TCEP which state that tmTCEP has a lower pKa value (4.5) and therefore works faster at low pH values, but there was no information where the authors...

02 March 2017 2,477 3 View

Is it possible to separate two antibodies bound to each other without interrupting binding to protein G resin?

I'd like to bind antibodies of species 1 to a protein A (or protein G) resin and to catch antibodies of species 2 that are cross reactive against species 1. At the end I have a sandwich of Resin...

05 June 2014 3,196 3 View

Advantages and disadvantages of sodium azide/thymol/thimerosal?

Typically most labs use sodium azide as a preservative, but there is also thymol and thimerosal that are also well-known preservatives. Is there a reason, why azide is used much more often than...

03 April 2014 4,836 11 View

What is best way to prepare a human erythrocyte ghost, and what is the best way to store them?

I need the blood group antigens of the erythrocyte membrane for my research and want to prepare ghosts out of EDTA blood. At the moment I just wash the erythrocytes in 0,9% NaCl, lyse in distilled...

31 December 2013 5,106 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Has anybody administered MK-2206 (an orally active, allosteric inhibitor of AKT) intravenously to the mice?

We aim to use MK-2206 to inhibit AKT in mice. However, a large body of studies suggests that it should be administered orally. I was wondering if somebody could tell me that is it toxic for the...

07 February 2021 6,340 1 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View