Is restriction enzyme PspCI CpG methylation sensitive?

More Nehir Mavioglu's questions See All

Does it make sense to run a multilevel linear model for data collected in different batches but all from the same university?

I applied an ANCOVA for my analysis and obtained beautiful results but the data were collected in three different batches, that is to say we have collected data from different people three...

07 August 2016 9,665 11 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?

17 July 2024 935 1 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

Isolation of Hydrogen Producing Bacteria?

any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...

13 July 2024 2,255 3 View

How to dilute essential oil from plants to assess the biological activity?

I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...

04 July 2024 8,100 4 View

Hello friends In simulating the drug with the liposome, which side of the liposome membrane is the drug placed on? The top leaf or the other?

Several times by placing a restriction on the upper part of the membrane with the aim of preventing the drug from being added from this side, we tried to keep the drug at the top of the leaf, but...

29 June 2024 9,688 1 View

Can I premix the reagent for restriction digestion and make a mastermix like PCR?

Hi everyone, I am currently trouble shooting my restriction digestion, so far it hasnt given me clear bands when I ran gel. I've been adding all reagents minus template, to make a master mix...

24 June 2024 1,171 3 View

Will this 3C Analysis work or are the fragments too small/close together?

I'm doing a 3C analysis shown in the picture linked and attached below with the Bait fragments labeled and the target fragments that will be tested for interaction with the bait labeled with...

02 June 2024 8,038 1 View

How to prepare 0.28 U/ml working solution of Acetylcholinesterase inhibition assay for anti-Alzheimer activity?

I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...

31 May 2024 6,444 1 View