T50 means the temperature that an enzyme lose 50% activity. Tm is the melting temperature of an enzyme. But the curve about T50 and Tm is highly similar. What's the specific differences between T50 and Tm? Thanks.
Hi there,
Tm is actually measuring protein unfolding process whereas T50 is measuring the loss of biological activity so the latter is only about the measurment of active site inactivation. Tm and T50 are not always correlated as a slight perturbation of the active site may be enough to fully inactivate the protein without significantely affecting the overall folding of the protein.
Hi there,
Tm is actually measuring protein unfolding process whereas T50 is measuring the loss of biological activity so the latter is only about the measurment of active site inactivation. Tm and T50 are not always correlated as a slight perturbation of the active site may be enough to fully inactivate the protein without significantely affecting the overall folding of the protein.
Hi. From another point of view (complementary to the previous answer), I would add that T50 may reflect the temperature-sensitivity of the particular enzyme state which is predominant during turnover, in cases where one of the various states explored by the enzyme during its catalytic cycle indeed is predominant during turnover, and where these various states do not have the same temperature-sensitivity (proteins may be more or less stable -i.e. have different Tms- depending on the presence or absence of their specific ligands, or in specific activation states)...
It's probably saying the same thing as Philippe Champeil in a different way, but the melting temperature of an enzyme can be substantially affected by the presence of a ligand, such as the substrate, usually resulting in an increased Tm.
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