To calculate the Km of an enzyme.
Dr.Tobias thank you for the information. I don't have access to the paper. I will search for similar articles. If you have access can u please send the article to me.
I am using a silicon nano wire in microfluidic channel to identify the some changes in parameters like voltage and current. but i am unable to interface them. the two physics run individually but...
10 January 2021 8,894 1 View
I am trying to select suitable element type (Solid or Shell) for impact analysis. I have tried with both solid and shell elements. The results are same for both element types up to certain impact...
08 January 2021 3,251 4 View
I am trying to simulate high velocity impact of rigid spherical projectile on a rectangular plate using Abaqus Dynamic Explicit. Particulars of Projectile Diameter : 12 mm Mass : 7.05g,...
04 January 2021 10,022 3 View
I am doing my research on a communication system. I need a Simulink model with MIMO OFDM anyone of you could suggest me to find the total OFDM transmitter and OFDM receiver Simulink design model
21 December 2020 3,709 1 View
What are the challenges in Machine Learning to integrate it with Mobile Adhoc Network
21 September 2020 3,881 5 View
I am simulating the compression test of the sandwich structure in Abaqus. How can I calculate? Please suggest me a method to overcome this problem. Thank you
05 July 2020 4,085 10 View
I am using maximum ratio combing and selection combing techniques for C-NOMA. I want to compare the results for both techniques and also suggest me any other alternative combing techniques which...
27 January 2020 7,557 10 View
I need to perform network simulation between wireless sensor network for my research work. Kindly suggest me which simulator is best and open source
21 January 2020 5,591 4 View
i am new to machine learning,how to find out a problem for research,could you guide me plz. Thanking you.
25 September 2019 5,119 5 View
Dear Sir, i need an IoT simulator tool that can be supported for simulating LoRa network. To my knowledge, i know Simple IoT simulator but it is paid version. Thank you,
07 July 2019 7,561 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...
16 February 2021 1,337 2 View
I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...
16 February 2021 4,224 3 View
I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...
14 February 2021 3,142 7 View
I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...
11 February 2021 1,328 3 View
Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...
10 February 2021 1,797 5 View
I am trying to do a clonogenic assay for PC-3 after irradiation. I have to synchronize my cells by serum starvation but there's a optimal time I have to do that before irradiation. been looking in...
09 February 2021 9,480 1 View
I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...
08 February 2021 733 6 View