To calculate the Km of an enzyme.
Dr.Tobias thank you for the information. I don't have access to the paper. I will search for similar articles. If you have access can u please send the article to me.
Sir, I want to work on the laser assisted machining (turning) of Nickel Based Super alloy. Please help in finding the laser assisted machining facility in India. Thanks and regards
02 July 2024 2,086 2 View
Can you please suggest it structural view and I want to calculate Power Dissipation, Delay Read SNM, Write SNM, write Access time, Read Access time, Read current. can any one suggest procedure...
15 February 2024 5,964 0 View
I have done parametric study in Ansys Material Designer by varying the volume fraction of carbon fiber. Now I would to have a look at stress distribution in the RVE for each design point. How can...
23 November 2023 6,063 3 View
RFID TECHNOLOGY PPT
11 October 2023 6,641 2 View
I would like to develop 2D open cell foam models which can be further used in the FE modelling. Could anyone please suggest any modelling tool? Thank you
05 September 2023 2,535 3 View
Dear RG community. I am trying to set up a fluorescent microscopic system for micro-PIV flow measurements. I am using a blue laser (473 nm) of 3mm diameter and I am expanding it using a 10X...
13 July 2023 1,973 3 View
I tried to troubleshoot it but still no use can anyone help me with this issue, ketone double bonds are missing in ligand map
02 June 2023 831 0 View
I need to develop a single module which should consists of multiple bluetooth modules .
27 May 2023 8,425 0 View
How the xrd values effect the medium , bulk and nano powders in the zinc oxide .
28 April 2023 5,891 1 View
I am teaching faculty is there any way to get IEEE membership at free of cost let me know and let me know any other memberships which exists which we can get at free of cost
11 February 2023 6,594 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View
What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.
21 July 2024 1,554 2 View
I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...
15 July 2024 9,025 3 View