Which technique may be the best among (a) Radiometric assay of an enzyme; (b) HPLC assay of an enzyme; (c) Spectrofluorimetric assay of an enzyme?

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Maria del Pilar Corena-McLeod

The answer depends on what you are trying to do. Are you trying to characterize the enzyme by size, by pI or by enzymatic activity? are you trying to study the binding site or just get an idea about its amino acid composition?

Sanjay Mishra

@Maria: Enzyme(s) catalyzing the reaction including the substrate and producing the product, both of them being fluorescent.

Are you aware of HPLC techniques to characterize the product or the substrate?

Thanks, Maria. I have the awareness of.

Apurva Kumar

The important thing is specificity, senitivity of the analytical technique and catalytic activity of the enzyme.