Methods in Enzymology
The answer depends on what you are trying to do. Are you trying to characterize the enzyme by size, by pI or by enzymatic activity? are you trying to study the binding site or just get an idea about its amino acid composition?
@Maria: Enzyme(s) catalyzing the reaction including the substrate and producing the product, both of them being fluorescent.
Are you aware of HPLC techniques to characterize the product or the substrate?
Thanks, Maria. I have the awareness of.
I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??
28 February 2021 2,153 1 View
How to conjugate two acids with amine linker?
18 February 2021 4,083 2 View
Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...
16 February 2021 1,389 2 View
Hi everybody, currently, I am working on the grid-connected inverter. I want to make my grid-connected PV system a PLL-less system. I have studied some reachers are implementing a virtual...
07 February 2021 6,078 2 View
Landsat, sentinel and USGS satellite images have varying accuracy for identification of urban built up dynamics ? Which one is best suited for India
04 February 2021 8,632 8 View
If condensation reaction happened between carboxylic acid and amine and amide is formed. The solvent for this reaction is toluene. After completion of reaction, how to collect product? It should...
03 February 2021 9,796 3 View
Hey, I want to know if I have fabricated some nanostructure and further I want to make a shell on the nanostructure by solution phase method by making it core-shell nanostructure. So, anybody can...
01 February 2021 9,966 4 View
My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...
30 January 2021 1,039 3 View
I want to publish my pilot study about corrosion in RC structures in any journal. Please help me to find good options.
25 January 2021 6,413 3 View
Can anyone please suggest to me whether I can able to check the fret efficiency between two proteins when one protein expressed after transfecting the cells, that might act as a donor(...
06 January 2021 6,958 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...
28 February 2021 6,477 1 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View
I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...
25 February 2021 2,388 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...
16 February 2021 1,337 2 View
I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...
16 February 2021 4,224 3 View
I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...
14 February 2021 3,142 7 View
I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...
11 February 2021 1,328 3 View