Hi,
I recently did RNA seq for my samples previously having done qRTPCR for a select number of genes. However as I was analyzing the data I noticed a few discrepancies. In my sample for QRTPCR the trend was Gene A and B are highly expressed and Gene C almost negligible expression. When I got back the RNA seq data, I noticed that Gene C is highly expressed and Gene A and B are very low in our samples. Can anyone explain the discrepancies? The primer sequences for QRTPCR were validated beforehand and taken from a previous publication. In cases such as this which result is accurate? For context, we are simply trying to understand the expression of some genes in our specific sample thats all.