i want to know why we are only doing double digestion in genomic DNA. also I want to know why we are doing both single digestion and double digestion in Plasmid DNA
When performing Karyotying using Peripheral blood sample, we add 250 microliters (collected blood into heparin tube) in to 5 ml PB-MAX culture media and incubate it for 72 hrs in CO2 incubator....
15 October 2023 8,533 0 View
When building the molecule in Gaussian 6, I need to know how to add a plus charge on the N atom.
05 December 2021 8,323 4 View
If not what are the possible options for DFT+U calculations?
11 September 2021 5,953 2 View
I am using secondary data (ROA, Total assets, Firm age) and use structural equation modeling for data analysis. Hope to use SMART PLS software. May I know how to enter data into the package? is...
17 August 2021 3,537 3 View
I am using an intel core i5 and 4GB RAM computer to calculate DFT (6-31G basis set) calculations for C, N, S based polymers. I have GaussView 6.0 and Gaussian 09 Revision-D0.1 SMP. So, does anyone...
28 July 2021 8,869 2 View
I am interested in polymers that contain "Benzathiadiazine"
18 July 2021 9,727 6 View
For my research need past solar radiation data in the Hambanthota district in Sri Lanka.
18 June 2021 8,593 10 View
Hello, Currently our group is trying to understand the use of Sentaurus for solar cell simulations. As per now we have gain reasonable knowledge on the process, but still having some issues with...
06 November 2020 3,803 4 View
I am working under bio-polymer micro beads and need to coat my beads to improve its durability. Can you comment about those coating materials?
06 September 2020 2,575 4 View
Hi, I am currently doing a research on Inborn errors of metabolism in Sri Lanka and for that, I need to develop a method to identify few SNPs and a single point deletion using allele specific PCR...
27 August 2020 2,575 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View