AFM for liposomes?

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Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

Comparing Liposome Size: DLS Measurement vs. SEM Observation with Air Drying ?

I have an issue with SEM imaging of liposomes. When measured by DLS, they appear to be around 120 nm, but when using SEM with simple air drying, they appear much larger, around one micrometer.

25 July 2024 3,395 3 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 5 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?

18 July 2024 9,831 0 View

Phospholipids storage conditions?

What is the protocol for storing phospholipids in chloroform solution? Should I divide the contents of a bottle into aliquots to prevent melting each time I take it out for an experiment from...

27 June 2024 2,138 2 View

I want to know where can i find n , k mesoporous peroviskite coefficients?

in this link refractiveindex.info i didnt find i work on a simulation comsol of mesoporous perovskite i want to know the properties optoelectric and n,k coff

09 June 2024 2,071 4 View

Can collagenase injection cause a reverse reaction leading to higher collagen deposition?

Can rats that were injected with collagenase NPs, show higher collagen deposition than their control untreated counterparts in histopathology examination!!!

11 May 2024 2,931 0 View

How to convert TiO2 powder into solution?

I had Titanium Dioxide powder & I need a specific answer for both points:- What is the most suitable solnavents used to convert TiO2 powder to TiO2 solution What is the most suitable methods...

30 April 2024 5,931 1 View

Statistical analysis using Graphpad Prism ?

Hey, I am wondering which tests I can do to analyze my results and if there is a better way to analyze my results. If I have multiple treatments in columns and I have just one donor in a row,...

30 April 2024 335 2 View

Should I test for RNA purity?

Hey, Usually, when I perform total RNA extraction, I directly synthesize cDNA and run qPCR without even analyzing or testing the purity of RNA. I am asked to use 15µl of RNA when synthesizing...

20 March 2024 1,380 2 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Ethylene glycol is newtonian or non newtonian fluid?

Some sources say it is treated as Newtonian as ethylene glycol has a higher viscosity than water, which affects the flow characteristics in simulations. But some also say it is non-Newtonian.

09 August 2024 2,111 2 View

Fabrication of narrow glass capillary (inner diameter 200~500 nm)?

I need a very narrow glass capillary for my research. How can I make a glass capillary with an inner diameter of around 200 to 500 nanometers?

08 August 2024 8,915 0 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...

06 August 2024 9,986 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

What are the shear and normal stiffness values of an LLDPE liner in 3D numerical modeling of a stockpile?

I am seeking experimental or applicable data for the liner (LLDPE) interface in FLAC3D numerical modeling of a large stockpile. Could you please recommend suitable references? The preferred data...

05 August 2024 3,665 0 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

Yun-Tzai Lee

Yes, you can. Make sure you are using the optical-grade with clear polished one.

https://www.tedpella.com/vacuum_html/Substrates_Supports_Wafers_Slides.aspx#16001-1

Janusz Rebis

Yes, any flat-parallel surfaces will be ok. Mica, glass, silicon wafer, and so on.

Aya Jab

I'm trying to cleave mica with a blade, but it breaks before it splits. Is there a specific way to achieve a clean cut with mica? Janusz Rebis