Cells that adhered look healthy, but I am worried about the number of floating cells in my culture. Is it normal to have a significant number of floaters in SK-BR-3 culture? I am using RPMI 1640 supplemented with 10% FBS, Pen/Strep, Sodium Pyruvate, and L-Glutamine. Thank you in advance.
Hi there,
SKBR3 culture medium from ATCC:-
https://www.atcc.org/products/all/HTB-30.aspx#culturemethod
In our lab we are culturing in DMEM with 10%FBS and all other components you have mentioned. As SKBR3 is adherent type RPMI1640 is not suitable for its growth.
Cheers
Hi Patrick
I know that it's is very long ago but may I know how you had resolved this issue..?
Because ai am also. ame across this problem.
Thanks in advance
Hi Shafi,
Try to culture them at high confluence, they tend to grow better when seeded close to each other. Other than that, make sure everything related to media is fresh.
In addition, I am not 100% sure that floaters are actually dead cells as I did not investigate much. There some breast cancer cell lines that can grow as both adherent and suspension, for instance MDAMB134.
Hope this helps;
Patrick
Thank Patrick
Thank for your suggestion.
I will try to culture in high confluency.
I am using everything fresh and recommneded one.
One more point some times in some flasks media changes into yellowish one just in one day having large no.of floating cell while in other media colour is same.
Have anyone came acroos this situation.
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