How to calculate the sample concentration with different dilution factor from standard curve?

A silly question!!! Pardon please. Usually a standard curve follows serial dilution with at least four points (especially in 5 point curve) in eight and I assume it reach to 10,000 dilution from...

21 December 2020 9,994 2 View

Where is Fluent 2020R2 "normalization" option?

I can't find the Fluent 2020R2 "normalization" option. Pic 1 (screenshot) is from a guide that a Professor sent me.

13 September 2020 5,620 1 View

How to get Ansys CFX to converge?

I have a problem with Ansys CFX. My runs aren't converging. I have attached a screenshot of a previous run. Any ideas on how to resolve this?

29 August 2020 3,387 14 View

How to destroy plant roots to become powder without damaging the polymer (solid sample preparation for FTIR)?

please give me advice. I want to trace polymer molecules in plant roots using FTIR. what is the correct method for turning my root sample into powder (solid sample) without damaging the polymer? I...

11 November 2019 9,006 4 View

How do I allow my essential oils to be miscible with my cell culture (SH-SY5Y) besides using DMSO?

I am working on the potential of essential oils on an Alzheimer's disease model using neuroblastoma cells SH-SY5Y. How do I dissolve my essential oils into the culture so it would not form a...

27 July 2018 351 1 View

Do you have questionnaire for diversity within groups, innovation, knowledge sharing?

I am searching the questionnaire for the diversity within groups, innovation, and knowledge sharing. If anyone could share me, it will be a big help for my PhD paper for degree requirement. Thanks...

25 April 2018 470 6 View

Why discrepancy between the results of deviance and pearson goodness of fit test in logistic regression?

I run a binary logistic model and had a results showing significant in deviance and non-significant in Pearson GOF test. I also tried HL stat and showed non-significant. How do I decide on that...

31 March 2016 9,860 4 View

How can I use the Lositan software to find out outlier loci?

It is pretty difficult for me to install Lositan and other necessary items such as Fdist2, Batik 1.7. Any advice on using Lositan, or any other methods for finding out loci of outliers?

05 January 2014 3,846 9 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View