Protein sample and mercaptoethanol?

Gehan Abdelaziz @Gehan-Abdelaziz

23 November 2024 1 1K Report

I forget to add mercaptoethanol to my SDS sample buffer of my protein sample.

I already heated my sample and stored it.

What should I do ?

Quentin Charras

Add it and reheat :)

Badges
Science topic

More Gehan Abdelaziz's questions See All

Comment publier un travail sur la plateforme ?

Publier des documents scientifiques

26 June 2024 7,343 1 View

Can people help me complete my questionnaire?

I am conducting research with title "Cost Overrun and Time Delay Prediction Using Machine Learning Algorithm: Application of a Hybrid Intelligence Model." and I need to collect responses on...

23 February 2024 3,544 1 View

Which is the essential book for learning more about singular elliptic and parabolic equations?

Dear Colleagues, On follow up on our new interest. Nowadays, research in singular elliptic and parabolic equations spans various areas of mathematics and applied sciences, including mathematical...

13 February 2024 5,088 0 View

How can I to use magnitic field in ansys fluent ?

I hope to get a clear answer on this matter

14 November 2023 2,926 4 View

Combined effect of both rotary inertia parameter (r_g/L) and shear coefficient in bending frequencies?

The shear correction factor (k) and the rotary inertia parameter (r_g/L) are both known to reduce the bending frequencies of Timoshenko beams. However, the effect of increasing each parameter on...

04 September 2023 9,195 0 View

I am trying to get Ionosonde Ne by using SAOExplorer, but it requires username and password?

I sent an email to the circled mail in the following screenshot without any reply. Is there any other way to connect to this website?

07 August 2023 5,527 0 View

What is the importance of applied mathematics in the field of industry?

importance of applied mathematics

25 July 2023 626 9 View

I am trying to read all the electron density profiles for one day by this code, but the result file is empty. can any one help me solve my problem ?

clc;clear; % Create an empty array to store the electron density data ne_array = []; % Loop through the files in the folder folder_path = 'D:\ionPrf_prov1_2020_002'; % replace with the path to...

02 July 2023 1,401 1 View

What can be the latest research gaps that need to be covered in 3D printing in construction field?

Kindly mention you points.

07 June 2023 794 3 View

Where can I find the answers for the word associates test (Read, 1993,1998) and the productive levels test (Laufer & Nation, 1999)?

Hello dear vocabulary acquisition researchers, I am looking for the answer sheet of the vocabulary depth test (Word associates Test) and the productive vocabulary levels test (Laufer & Nation,...

12 January 2023 8,247 4 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View