What is the difference between an oligonucleotide primer that used in PCR assay than that used in DNA Sequencing?

Oligonucleotide primers are used to generate bands corresponding to their own gene in PCR assay.

06 September 2020 2,066 7 View

Which company is good for DNA sequencing in Middle East?

I am looking for a good company in DNA sequencing in order to urge my students to cooperate with them and send their research projects to them.

11 October 2019 5,546 1 View

Arabic essay grading dataset?

Can you help me find an arabic essay grading dataset to be used for machine learning?

15 April 2019 3,998 3 View

What are the primer sequences used for APRIL-mRNA expression by RT-PCR?

Can anybody have the correct primer sequences to amplify APRIL gene (TNF-family) for reverse transcription of m-RNA as different articles use different primer sequences as well as the primers for...

26 March 2019 1,494 3 View

How can I judge my time domain measurment accuracy?

Model testing was conducted and we have measurements which varies with time of course such as (speed vs time), (motor current vs time), (force vs time), etc. I just want to measure the accuracy of...

08 January 2019 153 3 View

After submitting a revised manuscript (moderate revision was requested), what are the steps followed ?

15 December 2017 140 90 View

In a set of connected Nodes(routers), how can i measure the distance between them ?

In a set of connected Nodes(routers), how can i measure the distance between them in which i can predict how much time a Packet take to reach next node? Thank You.

06 April 2017 726 8 View

How to register Somatosensory Evoked Potentials (SEPs) using CED Signal software?

I was thinking of recording SEPs in patients with movement disorders. We are using CED signal software in our lab to register MEPs. It has been stated on the software website that it can be used...

28 January 2017 5,897 1 View

How can we estimate some missing values of a spring water flow?

12 August 2016 4,174 10 View

I want to ask about the practical way to transfer N fixing genes between different microorganisms ?

02 February 2016 4,961 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View