Can anyone help me out for C-reactive protein isolation protocol?

01 January 2014 1 383 Report

I want to isolate CRP from human serum. What is the best procedure to isolate it? With which compound does it bind most efficiently so that we can isolate it using affinity chromatography?

Saheem Ahmad

CRP isolation protocol. Can we use CNBr chromatography?

Badges
Science method

More Saheem Ahmad's questions See All

What is the best and cheapest protocol for monoclonal antibody production?

Please let me know the best way to produce monoclonal antibodies against any specific antigen. Please also let me know how much it costs for the production of monoclonal antibodies from...

04 May 2014 9,137 12 View

Transient Co-transfection of my gene construct having a GFP gene in it with the VSV-G envelope?

I want to do transfection in PG13 or in 293 gp cell line. I will be using Lipofectamine method. What should be the incubation temp, incubation time period and the amount of DNA for 10 cm petri plate?

03 April 2013 3,909 0 View

I am not getting the expected bands on an agarose gel after the digestion of the PCR products. I have performed PCR to add Mfe I and Age I Res. site onto the 5' and 3' ends of my gene.

My gene of interest is the T cell receptor gene (1383iTCR; 3 kb size) and I am trying to Ligate this gene into a pLVX-mCherry-N1 Vector (Lentiviral Vector; 8.7 Kb size). To match with the unique...

02 March 2013 2,125 5 View

Compensation of all wavelengths (colors) when analyzing for more than four antibodies in a single sample in a flow cytometry.

How can I do the compensation? What measures should be taken into consideration while doing compensation of 5-8 different antibodies?

01 February 2013 5,144 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View