I want to isolate CRP from human serum. What is the best procedure to isolate it? With which compound does it bind most efficiently so that we can isolate it using affinity chromatography?
CRP isolation protocol. Can we use CNBr chromatography?
Please let me know the best way to produce monoclonal antibodies against any specific antigen. Please also let me know how much it costs for the production of monoclonal antibodies from...
04 May 2014 9,259 12 View
I want to do transfection in PG13 or in 293 gp cell line. I will be using Lipofectamine method. What should be the incubation temp, incubation time period and the amount of DNA for 10 cm petri plate?
03 April 2013 4,026 0 View
My gene of interest is the T cell receptor gene (1383iTCR; 3 kb size) and I am trying to Ligate this gene into a pLVX-mCherry-N1 Vector (Lentiviral Vector; 8.7 Kb size). To match with the unique...
02 March 2013 2,250 5 View
How can I do the compensation? What measures should be taken into consideration while doing compensation of 5-8 different antibodies?
01 February 2013 5,263 1 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
or we can collaborate
01 August 2024 6,705 2 View