I want to analyze acetic, propionic and butyric acid in MRS broth (supplimented with inulin) fermented by probiotic bacteria.
There are similar kinds of studies suggesting different types of preparation for SCFA extraction from the sample. For instance, people have used different solvent (ether or hexane) and samples are esterified with acedified alcohol or directly loaded without esterification of SCFA. I would like suggestions and expertise of researchers working in this area.
There are two approaches:
A. Without derivatization: You need to isolate your compounds (Folch methods and further modifications are the best for lipid isolation). Then you inject in a proper column as a FFAP or other.
B. With derivatization: Again you have two options;
B1. Direct detivation: You do not need isolation. http://www.sciencedirect.com/science/article/pii/S0039914014004354
B2. Isolation and derivatization.
Then you inject these compounds in proper column as a CPSil88, BPX, VF23 or similar.
The main problem is these compounds are highly volatile.
http://www.sciencedirect.com/science/article/pii/S0039914014004354
Thanks Fakhrul and Luis for your inputs.
Since SCFA are highly volatile compounds so I went through direct injection method to avoid loss of the sample during preparation and I got good results using 10% SP1200 column.
Dear Namita,
if you have good results using that column then I suggest to isolate using Bligh-Dyer or Folch methods. Some people also have good results using the Hara-Radin method (isopropanol-hexane). Then inject as you usually do. You will only have to check for split and sample concentration.
Good work!
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