I have injected crude solvent extract from mycelial cells in C18 column.
No! It just means the molecule has retention capacity under those chromatographic conditions (mobile and stationary phases).
No. Perhaps by the uv-vis spectrum if you have a diode array detector. Still you will need to analyze a spectra database
And not even then, since there are some sidegroups that are invisible in the UV. Can you tell the difference between a sulphonate and a phosphonate?
MS will help but it would have to be CID and/or MRM.
No it is not possible to identify the compound, as there are thousands of compounds whose retention time may match with your compound. Secondly if your are using the UV PDA/ Mass detector you can get the idea of our unknown compound by observing the spectra.
Thank you for your answers.
I have used diode array detector, and have spectra. Will it help?
Fine, if you have little knowlege of probable structure of unknown compund, you can use woodward-fieser rule to predict is lambda max which should matches with the maxima which you have got on PDA by HPLC. https://en.wikipedia.org/wiki/Woodward%27s_rules
RT in HPLC establishes the condition which can be used to get pure compounds via PREPARATIVE HPLC while spectra methods such as NMR, IR and MS will help in characterizing the compound.
Hi, Yes I agree with Mr. Sunil, you need to do with LCMS, nicer if you can do with LC HR MS MS, and after that you can use some free on line MS data bases (Metfrag, MEtlin, GNPS etc) to predict your compounds
Sir Gunawan how good are these libraries? Are the identifications as good as NIST WILEY etc for volatile compounds? I have gone through a LCMS-MS report of an extract; more than 100 compounds detected but identification is very tentative.
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