What factors increase acidity of raw fresh water?

What is the reason of increasing acidity of raw fresh water, despite its high percentage of salts (suspended and dissolved), in addition to its low organic matter? Can atmospheric CO2 causes this...

14 January 2021 4,388 5 View

Can I use Scherrer formula to calculate the crystallite size/Coherent domain size of Li2O5Si2 (lithium disilicate) from XRD data?

I`m comparing two different samples containing lithium disilicate and under SEM (5000x) there was a noted increase in the particle size (1st sample 2.7 microns in length and the 2nd sample 5...

28 October 2020 5,905 6 View

Calculating Seeded Cells?

I want to do a cell based assay. This assay contains 5 uL of my cells (Dissolved in DMEM medium) and 5 uL of compound (dissolved in DMED medium also). Suppose I count the cells in my suspension...

19 October 2020 2,487 1 View

Is it possible to deliver hydrophilic drugs to lymphatic system after oral administration? what kind of drug delivery system could be used if (yes)?

Is it possible to deliver hydrophilic drugs to the lymphatic system after oral administration? what kind of drug delivery system could be used if (yes)?

01 July 2020 1,793 1 View

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14 June 2020 408 6 View

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26 February 2020 9,723 10 View

Where to find case studies for teaching and assessing studnets' understanding of agile project mgmt?

I am wondering if there are any case studies used for delivering and assessing students understanding of agile project management Scrum framework. Please feel free to share some of your agile PM...

05 February 2020 3,229 6 View

How can I obtain the pitch angular acceleration of Quanser Aero device using Simulink/Matlab experimentally ?

I conduct an experiment on the Quanser Aero device using Simulink/MATLAB, which is a twin-rotor MIMO system with two degrees of freedom; pitch and yaw angles. I want to obtain the pitch angular...

27 January 2020 3,709 4 View

Is there any to add the missing citations in Google Scholar Profile?

Google Scholar citation profile is considered as an important indicator across the globe. But the google scholar fails to identify citations and impact our profile. It would be nice to identify...

15 December 2019 9,726 3 View

Is there any difference between the output of the position accelerometer and the second derivative of that position for real-time implementation?

For real-time implementation for a device, I have theta which is the pitch angle and I have a built in accelerometer in the device. To get the angular acceleration of theta could I take the second...

01 December 2019 6,539 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View