How to change normal H silica gel to C silica gel for reverse chromatography.??
Hi!
I notice "reverse chromatography" is mentioned. Do you mean "reverse phase"? If so, this is a chemical derivatization that bonds the C18 chains as a silyl ether.
Type C silica is usually used as a desiccant.
Dear Jack Silver Silica gel reverse phase is used to isolate more polar components. My question is, how to change the normal phase silica gel into Reverse phase silica gel. To my knowledge we have to replace the OH from silanol group with C18, or C8 groups to make it reverse. But how?
It will be far easier to purchase silica derivatized with C18.
One way is to use a chlorinated silane to bond the C18 (Kirkland, 1970).
Alternately, halogenate the silica with thionyl chloride, then react this with a C18 (or C8) Grignard reagent (Locke, 1972; Brust, 1973). The silica will need to be heated to 300 °C to remove all water before the reaction, and all the HCl needs removed before the Grignard reaction.
- Badges
- Science method