I have a chromatography from a DNA sequence which is a heterozygote for a 3 pb deletion. This makes that for now on all the sequence have double peaks. An idea I have is to split it, so I have higher peaks in one sequence and smaller peaks in other, but I do not know if that is possible.
If anyone knows how to solve this problem, let me know. Thanks
Daniel Curbelo if you check, your sequence it is very lower from the 168 bo until 190 bp. When you analyse your senquence, you obtained a table with the value and the color blu or yellow. Which was your colour?
In my case this staff happen when I have some problem on the PCR before the sequencng.
But your PCR-product is from a pure culture or a sample?
Thank you some much for your response. This is a purified and amplified sample, but I strongly believe this happened because the individual has a deletion in one allele, so it is heterozygote for this deletion. I do not think it is a problem with sample contamination due to equal double peaks rate.
Do you know any method to split these two types of peaks? If not, I have to do it by hand, so it will be a very long task.
No because I work in microbiology and this happen when you sequence not pure fungal strain but for examples soil samples.
- Badges
- Science method