Variable readings at same time point and similar solutions
Hello everyone, I am planning to do a cloning experiment. Can anyone suggest what is the minimum amount of insert DNA and vector DNA required for a 1:7 ligation reaction ? The size of my insert...
17 April 2024 5,845 2 View
hi everyone, i am trying to figure out why the gene inserted is not amplifying after cloning. I have inserted a mycobacterium-specific gene into pET 32a plasmid and transformed the ligation...
06 April 2024 7,691 8 View
I require a cucumber leaf disease dataset with different diseases for an image classification problem. How to access the cucumber leaf diseases scan dataset?
03 June 2022 6,151 0 View
I have 8 variables in my study, out of which 4 are my experiment variables and 4 are control variables. Based on control variables as my ground truth, I get 2 clusters as optimal (based on...
22 May 2022 2,765 2 View
I am trying to cluster a few categorical & continuous observations using an unsupervised cluster algorithm. I have used two-step clustering since I have categorical predictors! Could someone help...
20 May 2022 1,137 3 View
If both 'avoidance' and 'response inhibition' mean a context-appropriate response, what could be difference between them in the field of psychology?
14 September 2021 1,930 3 View
I have collected a 30-minute HRV during a stressful task for two different groups. To compare the variations, i have seen many papers average the hrv over the collected time. Is that a right...
03 October 2019 9,316 1 View
it's important to know that we are using the inactive elements of Cs, Sr, Co
25 July 2024 1,553 2 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
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02 July 2024 918 2 View
spectrophotometric determination of iron and copper
04 June 2024 1,720 0 View
Hi everyone, has anyone else experienced an 'Error 8008' with their NanoDrop 3300 (image attached), on a laptop which it previously worked fine on? I have tried uninstalling the software and...
02 June 2024 9,025 3 View
I want to determine the conjugated drug to IgG ratio by nanodrop. I know the extinction coefficient of IgG and drug by M-1cm-1 but all the unit in the nandrop program is based on g/ml and so on...
15 May 2024 9,097 4 View
I manually extract DNA using a modified Sambrook protocol (phenol-chloroform), from tissue samples (skin or muscle) of stranded animals even at a high state of decomposition. When I take Nanodrop...
05 May 2024 5,588 1 View
Methods are based on Chel-Guerrero and colleagues'Phytochemical Profile, Toxicity, and Pharmacological Potenti... . We use a 1M Tris buffer with pH of 6.8, with Bovine Serum Albumin as the...
25 April 2024 8,802 0 View
Hello, I'm trying to elaborate a denaturing 12% polyacrylamide gel (19:1) with urea 8M in order to visualize the presence of plasma total miRNAs, I'm using TBE buffer, my extraction (Trizol)...
22 April 2024 1,740 3 View
I ran PCR using COI universal primers on DNA extracted from lice. I added 25ul of 2x master mix, 5ul template, and 1ul each of 20uM F and R primers, with the remaining volume made up with DW to a...
20 April 2024 1,565 4 View
