Variable readings at same time point and similar solutions
I have collected a 30-minute HRV during a stressful task for two different groups. To compare the variations, i have seen many papers average the hrv over the collected time. Is that a right...
03 October 2019 9,174 1 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I measure the wavelength of material in a spectrophotometer and it peaks at 275 and its absorbance is 100. I mix it with an adsorbent and it adsorbs the first material, but when I measure the...
16 February 2021 7,515 5 View
I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!
11 February 2021 7,273 3 View
I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...
08 February 2021 2,673 1 View
Dear Researcher Respectfully, I am working on drug delivery. I synthesized Zn-MOF. I am going to load Doxorobicin on it and the study the derivery behaviour. I tried several times to load. but I...
07 February 2021 748 2 View
Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...
06 February 2021 9,370 7 View
Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...
05 February 2021 292 3 View
I have prepared gold nanoparticles using the citrate reduction method in the lab. I need to characterize them using UV-Vis and DLS, by how much should I dilute my sample for using it in the...
26 January 2021 530 5 View
I want to calculate the concentration of DNA in an unknown sample. But it's buffer has a high concentration and our nanodrop don't accept that as a blank of dsDNA. Thus I dilute the buffer with...
23 January 2021 9,440 2 View
I am working on PVA-GMA hydrogel that is a really strong polymeric structure like rubber. Because I am in the middle of analysis, it is difficult to dissolve the gel using a common solvent. I...
20 January 2021 3,501 2 View