I have applied a general protocol. In short:
20uL of serum were added to 8 volumes of a cold TCA+ Acetone solution. After a light stirring I incubated for 90 minutes at -20 followed by centrifuge at 15,000 rpm for 20 minutes. The pellet was washed with acetone and then lyophilized.
The coloration revealed that the albumin was not completely removed from the serum, and the pellet did not resuspend properly. I tried resuspending the pellet in Laemmli Sample buffer.
Can anyone help me?
There are kits for albumin removal from serum samples. Here is one:
https://www.thermofisher.com/order/catalog/product/85160
Unfortunately I do not have the possibility, at the moment, to buy kits and since I have to process many serums I decided to try the protocol that I indicated.
Isopropanol + TCA gives better results
Article A Novel and Cost Effective Method of Removing Excess Albumin...
- Badges
- Science method