Hello,
I am trying to figure out sprouting quantify with using FIJI skeletonize plugin on CD31 IF staining heart tissue samples. The problem is afterwards using this plugin, I have 2 different table; Result and Branch information. Which dataset fit for sprouting analysis to compare of treatment vs control tissue samples?
Branch information includes these datasets; Skeleton ID-Branch length-V1 x-V1 y-V1 z-V2 x-V2 y-V2 z- Euclidean distance-running average length-average intensity (inner 3rd)-average intensity
Result table includes; branches-junctions- end point voxels- slab voxel- average branch length-triple points-maximum branch length
Thank you.
There are several parameters useful for Sprouting quantification of CD 31 IF are Vessel density ( no of vessel / area) , Vessel length per unit area, sprout number and sprout area
Vessel density are important parameters for sprouting quantification apart from vessel length sprout number
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