Good evening,
I wanted to ask you about something I have been observing. I work in a laboratory that does cell biology and molecular biology and we usually have some reagents and kits in the refrigerator at -30°C. I have noticed that some of these have a precipitate, one is a VILO buffer, and another is a centrifuge tube with antibiotics.Can anyone explain me the cause of this, please. Can these reagents still be used or should I get new ones?.
Thanks in advance.
Hi Adolfo Ubidia-Incio
It happens when the solution contains some SDS component or some other solutions which eventually precipitate when you keep below -15 or -20 degree. Nothing to worry about that. Just take out from the fridge, thaw and put some moments in heat block then the precipitant will disappear. It will not again precipitate until you put back at -20 or -30 degree.
Thanks
When you thaw, try leaving the tube or vial at room temperature and vortex, the precipitate should redissolve. Should not worry about that.
Hello, if no precipitate at room temperature you can still use it.
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