We are working on a new compound for Alzheimer disease and use THP-1 cell line and Amyloid beta 1-42 from US peptide as stimulator. Since, US peptide does not provide this Amyloid beta anymore we have to buy Amyloid beta from another company but I am not sure can I peruse the rest of my experiments using another Amyloid beta or I should redo all of my experiments including PCR using the new one. Either way, does anyone have any idea which Amyloid beta 1-42 normally is being used for this disease and cell line?
Special thanks in advance for your kind help
Dear Davoud,
As I have seen in the literature, AB-42 is the most abundant amyloid beta in diffuse plaques.
Of note, ketamine and xylazine are also being used in order to develop AD in animals.
For the case of brand change, In my opinion, you can make a comparison in two different brand treated groups and then decide to redo your experiments or not.
Best,
Hi Davoud,
This won't be much of a help to you, just a same-pinch. We also have the same problem. I use Amyloid beta 1-42 in neuroblastoma cells and the US company (ERI) no longer provides the peptide. We are searching and testing samples from different suppliers but still didn't find a suitable match. Will have to optimize the doses to be used that would give the same toxic effect as before. I don't think re-doing all experiments is practicable
Good luck in finding a suitable supplier.
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