Gromacs Protein com Ligand com distance, which is the right selection syntax for gmx distance?

I would need to check the distance between the two centers of mass of a Protein and of an organic Ligand (the resname and the resnumber of the ligand are both known), inside a system containing...

22 April 2020 3,696 4 View

How to isolate keratinocytes from fibroblasts in a co-culture using medium?

Currently I'm culturing primary human fibroblasts and keratinocytes from a skin punch in medium (DMEM with 20% FBS and 1% P/S). I would like to culture these cells separately, but I was wondering...

01 March 2020 9,425 3 View

Alternative for Qvotek microinjection needles?

A while ago the website of Qvotek went offline, and their supplies haven't been open for ordering. We were using their custom 5 micron micropipettes/needles/tips for microinjections in zebra fish...

11 November 2019 2,566 3 View

Measuring leadership Effectiveness?

I am looking for an existing tool that would assist in measuring sustainable practices on leadership effectiveness .

18 August 2018 3,640 4 View

What is the potential for individual giving programs for organizational sustainability in Africa? studies?

I am interested in finding out studies conducted or papers presented on individual giving in Africa. Please share. Best regards

13 August 2018 2,971 1 View

Thoughts about professional certification such as CISSP, CSSLP, CISA, CISM, etc. as job requirement for professors.

Do you feel that computer science and information systems programs should also require professors to have professional certifications in addition to the normal publishing requirements for tenure...

06 October 2013 1,633 6 View

Publishing in different journals vs Publishing in similar journals

For the same number of papers, is it better to publish in different journals (averaged fewer papers per journal) or publish in a few journals (averaged more papers per journal)? Does anyone have...

27 August 2012 1,223 72 View

Please can anyone refer me to a site that can give me a step-by-step procedure for artificial neural network analysis in Matlab?

01 January 1970 2,939 9 View

How can a user perception be measured for design of Virtual Learning Environments?

This question is posed in the context of designing computer simulations of real life learning scenarios using game engine platforms. Can the user experience a real life experience in the virtual...

01 January 1970 6,196 6 View

Sympathy different from empathy?

How do these two compare and/or contrast. A lot of confusion seem to arise during usage. Specific examples in usage are welcome, please.

01 January 1970 9,604 6 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View