I'm looking for a stain that can be used in 96 well plates (i.e high throughput) for quantitative assesment of tau tangles in iPSC derived coritical neurons. I've considered Thioflavin S/T but my impression is that the cells need to be fixed and on cover slips so not appropriate.
Actually, there is a study using Thioflavin S/T in 96 well plate.
https://www.jove.com/video/51537/in-vitro-aggregation-assays-using-hyperphosphorylated-tau-protein
Sui D, Liu M, Kuo MH. In vitro aggregation assays using hyperphosphorylated tau protein. JoVE (Journal of Visualized Experiments). 2015 Jan 2(95):e51537-.
In part 3, it states
3. With-dye, Continual Mode Assay on a SpectraMax M2 Plate Reader
NOTE: This assay differs from the previous one in that the fluorescent dye ThT or ThS is included in the aggregation reaction. This allows continuous measurement of the same set of reactions. Because of the repetitive use of the reaction, this method is better done with an automatic multi-well plate reader (as shown below the operation of SpectraMax M2). A regular fluorometer also works but the frequency of measurement of fast aggregation reactions is somewhat limited, due to the manual nature of the operation.
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