Hi,
what marker do you suggest to label recycling endosomes Rab11, Rab11a or Rab4?
thanks a lot
Have some idea here..Best..
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829897/
You can use antibody against Rab11 A/B to label slow recycling endosomes. Rab4 is thought be involved in fast recycling. When we normally say recycling endosomes it refers to the slow recycling endosomes. Whether you should use Rab11A/B depends on the cell /tissue type that you are using. Please check online database for the expression level of the Rab11A/B in your system and then use the right one.
I think many people use transferrin receptor to label recycling endosomes.
Pathways and mechanisms of endocytic recycling
Barth D. Grant* and Julie G. Donaldson§
Barth D. Grant: [email protected]; Julie G. Donaldson: [email protected]
*Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854,USA § Laboratory of Cell Biology, NHLBI, NIH, Bld 50, Rm 2503, Bethesda, MD 20892, USA
Abstract
Endocytic recycling is coordinated with endocytic uptake to control the composition of the plasma membrane. Although much of our understanding of endocytic recycling has come from studies on the transferrin receptor, a protein internalized through clathrin-dependent endocytosis, increased interest in clathrin-independent endocytosis has led to the discovery of new endocytic recycling systems. Recent insights into the regulatory mechanisms that control endocytic recycling have
focused on recycling through tubular carriers and the return to the cell surface of cargo that enters cells through clathrin-independent mechanisms. Recent work emphasizes the importance of regulated recycling in such diverse processes as cytokinesis, cell adhesion and morphogenesis, cell fusion, and learning and memory.
Recycling endosomes are key platforms for endocytic recycling that return internalized molecules back to the plasma membrane. To determine how recycling endosomes perform their functions, searching for proteins and lipids that specifically localized at recycling endosomes has often been performed by colocalization analyses between candidate molecules and conventional recycling endosome markers. However, it remains unclear whether all the conventional markers have identical localizations. Here we report finding that three well-known recycling endosome markers, i.e., Arf6, Rab11 and transferrin receptor (TfR), have different intracellular localizations in PC12 cells. The results of immunofluorescence analyses showed that the signals of endogenous Arf6, Rab11 and TfR in nerve growth factorstimulated PC12 cells generally differed, although there was some overlapping. Our findings provide new information about recycling endosome markers, and they highlight the heterogeneity of recycling endosomes.
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