Dear Sinam,
U73122 Solubility:
U-73122 is soluble in chloroform (10 mg/ml), DMSO (0.9 mg/ml), ethanol (0.7 mg/ml), and is insoluble in water. Higher concentrations in DMSO (2.6 mg/ml) and ethanol (5 mg/ml) can be achieved by heating.
https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/1/u6756pis.pdf
Stability:
It is very stable if kept in tight containers and protected from light.
Rafik
Dear Rafik,
Thank you for your valuable reply.I have gone through the sigma data sheet about U73122. But I am still confused regarding solubility and stability. Because santacruz and millipore have recommended the following preparatory instructions:
Preparation of Working Solutions:
1. Dissolve U-73122 in Dichloromethane or Chloroform .
2. Aliquot amount needed for a single experiment into suitable vials.
3. Evaporate the methylene chloride or chloroform using nitrogen.
4. The result is a film of U-73122 that can be dissolved in DMSO (5mM), ethanol (2mM in 100%) or an aqueous medium that contains serum or serum albumin (1-10 mg/mL), which forms a complex with U-73122, thus eliminating the need to use an organic solvent to deliver the compound to the cells.
That is why I am conscious about directly dissolving the U73122 in DMSO and Ethanol and how long this dissolved stock solution can be stored?
Dear Sinam,
This compound is very stable in ethanol and DMSO. You can store these solutions in tat low temperatures 2-8 C, however protect from light since it contains an amine function which can be oxidized upon exposure to light. You can cover the flask containing the solution with aluminium sheet and store it at 2-8 C.
Good luck,
Rafik
Dear Rafik,
I had made 5mM solution of U73122 in 100% DMSO, and aliquoted in different eppendorfs but this DMSO solution is very unstable and not even stable for 1 month. Freshly prepared solution shows good activity but after storing at -20 degree , there is the formation of precipitates. What should I do to increase its stability ?
- Badges
- Science topic
- Similar topics
- Medicine
- Oncology
- Cancer Research
- Cancer Biology