I am writing a research budget for my research proposal. I wish to use the Shimadzu Nexera X2 and Sciex QTRAP 6500+ system for the PFOS analysis. How much amount of N2 gas does it consume per sample analysis? For 100 sample analysis, how many cylinders do I need to include in my budget? Please help me if someone knows.
The Shimadzu Nexera X2 UHPLC and SCIEX QTRAP 6500+ LC-MS/MS consume ~30–35 L/min of nitrogen for nebulization, drying, and curtain gas. With a 20-minute runtime per sample, nitrogen consumption is ~700 L/sample. For 100 samples, this totals 70,000 L (70 m³). A 50 L cylinder at 200 bar provides 10,000 L, requiring ~7 cylinders. To account for losses, budget for 8 cylinders. Alternatively, liquid nitrogen generators reduce costs.
Running HPLC-MS/MS on cylinders is not very professional and cost effective. Use either liquid nitrogen tanks and evaporation units or devices that produce nitrogen from air. - consumptions of all machines is similar I believe, but can depend massively on the HPLC-Flow. Capillary LCs will use very little and 4 mm columns excessively much. The current best proce approach is run 2mm UPLC columns and use 10 min UPLC cycle time
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