Hi there, I am hoping there is an easy fox here. A student of mine is using the Lowry method to quantify total protein content in microalgae biomass. In running the assay however, he accidentally prepared his first batch of Lowry reagent D in a much stronger solution of sodium hydroxide than intended, i.e. 1 M not 0.1 M. Used to solubilize the microalgae proteins, I just wonder if this high concentration of alkai will denature the proteins and for example, affect their optical absorbance properties for spectrometer readings? Many thanks for your expert assistance.

Kind regards

Linda