Hi
I am working with human bone marrow derived stem cell. I am trying to lyse and wash the RBCs in the sample. Any suggestions to what to use and if there is an exact protocol of how to do it? Thank you
Diana Baxter
There are RBC lysis buffers available commercially. Alternatively, you can make your own (0.8% NH4Cl, 5% PBS, in water). The main thing to remember is that you need to stop the lysis buffer before it starts lysing your other cells. Generally, depending on the number of cells, wash your cells with eg. PBS or HBSS, then resuspend them in the lysis buffer. Wait for 4 minutes, then stop the lysis by adding 2-3x volume of media+FBS. Centrifuge the cells. If you suspect there are still RBC remaining, you can repeat the lysis.
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Chemistry
- Biochemistry
More Mustafa Hammad's questions See All
Similar questions and discussions