How can i get proper bands in sds page for type 1 and 3 collagen ?

More Mahesh Prabu Elangovan's questions See All

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

What is the difference between positivism and post positivism?

Your comments are being sought in regard of research methodology. Could you explain the meaning of postivist and positivism. How post positivism is different than the positivism?

26 December 2020 8,614 4 View

How to find APC without open access choice in hybrid journals. ex springers ?

I was going through springer journal websites and only APC for open access is given. But I would like to know the cost of publishing without open access choice in hybrid journals. So how to find...

18 September 2020 5,861 3 View

Dear all, Can any one say how to calculate convective mass transfer coefficient and evaporative heat transfer coefficient in fluent?

Is it possible to find in fluent? Any other method is there?

10 September 2020 5,492 3 View

Can we calculate the average value of the particular sub-region of Raster TIFF image?

1) I have calculated various Drought Indices for all the 26 Mandals of the Warangal Region India. Can I calculate the average value of a particular drought index in any sub-region directly by...

19 April 2020 1,127 7 View

Is it possible to establish the relation between the Remote sensing based Vegetation Index and on field Crop area sown Data?

I calculated vegetation indices (MSI, NDVI, NDWI, NMDI, SWCI) for the Warangal region of India from 2014 to 2019 (May month) by using Landsat8 data. I got the on-field data of crop area sown from...

17 April 2020 1,943 2 View

How to apply deep learning for estimation of various parameters in single carrier system?

Parameters like carrier frequency, symbol timing offset and symbol rate estimation in a single carrier system.

23 February 2020 2,980 4 View

Cosine Similarity between columns of two dataframes of differing lengths ?

Hi All I have text column in df1 and text column in df2. The length of df2 will be always > length of df1. I want to calculare cosine similarity for every entry in df1[text] against every entry...

30 December 2019 7,453 1 View

How to perform antiferromagnetic DFT calculations?

There are three types of antiferromagnetism, namely A , C and G type. I want to perform DFT calculations with these types of antiferromagnetism using either Quantum Espresso or Wien2K codes in...

24 October 2019 7,175 3 View

Standard dose of lead acetate creating testicular toxicity and route of administration?

Hello everyone i read number of articles but everyonechoosed diffirent dossage and route of administration but which is the best dossage creating testicular toxicity in rats and route of...

25 September 2019 7,177 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View