How can i get proper bands in sds page for type 1 and 3 collagen ?

More Mahesh Prabu Elangovan's questions See All

UDF code for 2dof forced osciallation of cylinder?

Hello I am trying to simulate 2dof cylinder for VIV analysis and I used an FDM method but the cd and cl values i got are so high and the damping is not coming into picture i tried and modified the...

17 April 2024 4,597 0 View

How many wild type of rodents for study of zoonotic diseases?

Please suggest how many rodents required for any study wild type rodents

04 April 2024 4,483 0 View

Arruda-Boyce(8-chain model) UMAT availability?

Can someone provide Arruda-Boyce(8-chain model) UMAT code for Abaqus?

12 March 2024 2,854 0 View

Is there any subroutine in Abaqus that I can use to delete the whole part?

I can delete the elements, but can we also delete the part when the strain in an element of the part exceeds some value?

06 March 2024 1,245 3 View

Does anybody know the copy number pf pET28a plasmid?

I am looking for exact copy number of pET28a plasmid. A citation would be great. Literature search only shows that it is low copy vector, but I haven't found any papers that mention the exact copy...

26 February 2024 2,358 1 View

What are the alternative methods to measure the filler dispersion in a rubber composite other than dispergrader?

Hi everyone, I would like to seek some answers from you for my question above mentioned. If you know something let me know. Cheers

23 February 2024 2,397 2 View

Can we cultivate a globally conscious generation to ensure the survival of humanity in the face of potential extinction events?

In this research, I propose a visionary approach aimed at cultivating a united and forward-thinking human generation dedicated to ensuring the survival of our species. The strategy involves...

30 December 2023 2,085 1 View

Which research papers for initial level gear train design is there?

research papers for initial level gear train design is

13 December 2023 3,065 2 View

What is the requirement for gear train design?

wanted to know What is the requirement for gear train design & gear design & devlopments

07 December 2023 239 2 View

How to avoid Pin holes and micro porosity issue in thermal Spray and Induction Fused coaitng?

•Coating method- Thermal Spray and Induction Fused. (Thermal coating by metco 6P-2 torch and fusing by induction heater.) •Part material- AISI 1035 Steel Plunger (4.5'' Dia.) coated by Water...

06 November 2023 7,444 1 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,370 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,802 3 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 947 4 View

I found the zeta potential of exosomes in DLS and it was -8mv. So is it correct or not ?

What should be the zeta potential of bovine milk exosomes

25 July 2024 7,376 5 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,936 0 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,662 6 View

What is the cause of this aggregation just bellow 70 kDa mark?

Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...

21 July 2024 5,127 4 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,904 0 View

Less substrate signals towards the last lanes of the Native PAGE?

I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...

17 July 2024 6,210 4 View