Hey I have my gene of interest in a pacebac plasmid. the cells we have are DH10bacs and not multibacs. Can I go down this route? or should i order?

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John Odonnel

No, you cannot go down this route. The pacebac plasmid is designed to be used in multibac cells, not DH10bac cells. The pacebac plasmid contains a replication origin that is specific for multibac cells. If you try to use the pacebac plasmid in DH10bac cells, the plasmid will not replicate and the gene of interest will not be expressed. You should order a different plasmid that is designed to be used in DH10bac cells.

Wali Mohd Dar

It depends on the gene of interest and the type of experiment you are running. Generally speaking, if you need to perform a specialized experiment, it may be necessary to order a multibac plasmid. If you are just performing a basic experiment and your gene of interest is compatible with DH10bacs, then you can go down this route.