We need to observe a compound (curcumin) in the footpad of a mouse. Solvents used for histopathology are formaldehyde and nitric acid. In addition, we have to decalcify the bone in order to do the analysis. Is there any chance that the test compound (a depot of curcumin) may be degraded or dissolved of the tissue being exposed to organic solvents? The idea is to observe where the substance remains without being affected by the treatment of the sample.
Hi Matthew, thanks. We want to use formaldehyde and basic EDTA or nitric acid to decalcification. Curcumin is stable in aquous and acidic environments but we don't know maybe those solvents could affect the movement of the deposit of curcumin within the tissue. Thanks.
Is Curcumin soluble in acetone? If not, acetone is a standard replacement for alcohol as a dehydrating agent and does not need a transitional step such a propylene oxide to get it into any of the various epoxide embedding formulas.
It appears that a protocol of decalcification with formic acid and nitric acid is useful...
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