11 November 2015 13 7K Report

I have bought Sigma Isoproterenol Hydrochloride (I6504). I have dissolved it in KRBH buffer (pH-7.4) to make it 50 mM stock and stored @ -20. I used 10 micromolar conc. to treat adipocytes for 1-2 hr. But I am not getting any desired effect. One protocol used 1 mM HCL to dissolve isoproterenol.

http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PL00000265.pdf?pid=PL00000265

What I have done wrong?  Please suggest. 

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