Does any know the difference between psPAX and PAX2 LV plasmid?

I want to know which one is more suitable for dendritic cell transduction. I see that in: https://www.cell.com/cell/pdfExtended/S0092-8674(15)00829-6 PAX2 plasmid is used for dendritic cells...

31 December 2018 3,696 3 View

Is there a difference between human and mice codon optimised sequences?

I have a Cas9 plasmid which is human codon optimised. Will there be any difference in Cas9 protein expression if this is expressed in mice?

07 August 2018 5,808 0 View

How to add +/- signs in front of calculated mean value in Excel?

I'm not referring to error bars. I want to add +/- signs in front of my mean (just like in a paper), without having to go to insert symbols and inserting them individually. Are there any keys to...

10 November 2013 8,421 9 View

Is SOD and CAT activity present after 24h of autopsy provided its stored at -20C?

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09 October 2013 5,827 1 View

In which solvents are beta-carboline alkaloids soluble?

I tried it with water, 5% DMSO but it seems to insoluble in those solvents.

09 October 2013 6,216 7 View

Why are standards for phenolics dissolved in methanol for HPLC?

Why are standards for phenolics dissolved in methanol for HPLC?

09 October 2013 350 9 View

Identifing the epididymal luminal protein/s in lizards?

08 September 2013 2,651 4 View

TLC trouble shooting

I'm running TLC with silica sheets. As the solvent travels quarter way up its movement changes such that we no longer see a straight line of solvent but rather a squiggly line. This largely...

08 September 2013 7,048 15 View

What are your thoughts on my observations of STZ in fed and fasted conditions?

What should be the nutritional status of wistar rats before the injection of STZ.? I came across this very interesting paper titled 'Streptozotocin is equally diabetogenic whether administered to...

07 August 2013 6,918 1 View

what does 30% w/v of H2O2 mean?

what does 30% w/v of H2O2 mean? does it mean 30g of H2O2 is present in 100ml of that solution?

07 August 2013 529 17 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View