With the ChIP protocol there are two washes performed, one with a buffer that has the following ingredients:

Tris-HCl pH 8

EDTA

NaCl

Triton X-100

SDS

and the other which has the following:

Tris-HCl pH 8

NP-40

LiCl

sodium deoxycholate

What is the purpose of changing the salt used?

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