I am attempting an extraction of garlic poly sulfides from fresh, aged, or boiled garlic samples suspended in water. (5 grams chopped bulb in 5 ml water) Two pulls with 5 ml chloroform yielded a colorless organic layer but an unchanged yellow aqueous layer. A single pull with hexane gave similar results.
I believe the poly sulfides should be yellow, do they become colorless upon leaving the aqueous phase? If indeed the yellow components are the poly sulfides why are they remaining in the aqueous layer? Is there a better procedure for organic poly sulfide or allicin extraction?
Well, these compounds are actually somewhat soluble in water, and only sparingly soluble in hexane and chloroform. I'm not sure what your end goal is, but here's an NSF link http://www.nsf.org/business/ina/garlic.asp
Allicin oil is light yellow liquid, with strong garlic odor, insoluble in water, soluble in ethanol partly. Although the main component in garlic oil belongs to thio ether compound, the chemical property is stable. Under non-strong acid environment, it is not easy to decompose in 120C. However, if it is exposed to ultraviolet radiation for long time, it may induce decomposition. But Allicin powder is premix compound formed by garlic oil adsorbing some carrier. White and floating thin powder.
And dially polysulfides are colorless... Why don't you check by TLC if your aqueous/organic layer content your compounds?
I would say that allicin is less soluble in water than dially sulfides. I used to extract them by diethyl ether, as suggested in the synthesis by Yuan et al.
http://link.springer.com/article/10.1007/s11771-006-0079-4#page-1
Good luck!
Highly pure samples of the diallyl polysulfides are available commercially from
LKT Laboratories
2233 University Avenue
St. Paul MN 55114
Ph: 888-558-5227 FAX 651-644-8357
Easier than separating them out yourself, but not inexpensive.
Daniel
And would you send me please for allicin extraction on garlic if its any simple method is there ??? I need urgently and I could not find simple and easy method!
Ethanol is best, non toxic n low boiling point. Forget about lab scale, buy commercial unit from China.
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