I need to isolate early endosomes in a easy fashion. It is possible?
What is the best method for correcting task-fMRI head movements in a case study with SPM (1st level analysis only) ? My main problem is the presence of significant activations (clusters) which...
05 June 2024 3,700 0 View
Tungsten carbide nanopowders under examination were identified by a residual amount of carbon, with the characteristic D and G bands. However, the peak at 2000 cm-1 is rarely found in carbon...
26 January 2022 7,024 3 View
The elastic modulus of a single glass fiber results 75 GPa from a tensile test, should be the modulus of a bundle (parallel glass fibers) comparable with the single one?
17 January 2021 7,224 1 View
Good day, I am working on the molecular detection of Rubella virus. For the positive control I tried extracting RNA from the MMR vaccine(Tresivac). I have not seen any RNA after extraction. Its a...
03 November 2019 9,965 1 View
My positive control (conc: 4, 8, 12, 16, and 20 ug/mL); acarbose had an unexpected result, my least concentration for acarbose is 4 ug/mL and it actually showed the highest %inhibition for alpha...
07 May 2019 3,040 7 View
We are using the APSlifetech extraction kit to extract DNA from whole blood samples kept at -70°C for a year and half. The problems is that we are not getting DNA, and if we have it is of Low...
29 October 2018 6,234 3 View
Hello everybody. Acctually I am work with rotenone and T98g cells. Surprisingly, in a moment the rotenone does not kill my cells. I have try with other rotonone, I have change the confluence, the...
23 June 2016 1,920 3 View
data scientists
07 April 2016 2,309 3 View
We try to performed some experiments in vitro, including transfection, in testicular Leydig cells
16 March 2015 5,832 3 View
From my samples, I can get a maximum of 5-10 micrograms of total protein. I need to quantify a protein with low expression. Standard WB does not seem to be the appropriate method. Someone have...
04 April 2013 7,499 20 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...
30 July 2024 4,267 2 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...
28 July 2024 540 2 View
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...
24 July 2024 6,592 5 View
Are both ways acceptable, or is there any convention to them? Thanks.
22 July 2024 6,988 4 View