I'm working with both facultative anaerobes and obligate aerobes. I was wondering do SIM and/or MIU tests work if the bacteria are aerobic? If not what tests should I do in order to find out whether my bacteria are motile? Hanging drop method doesn't work for me, I've also tried simple wet mound method with some dye to help visualization.
Sharareh Harirchi
This paper may be helpful. Also, you can do SIM in a rubber stopper tube or add paraffin after inoculation to the SIM.
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Suhad Mohammed
https://microbeonline.com/tests-bacterial-motility-procedure-results/
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Maurice Ekpenyong
This should help, I believe. They are very commonly exploited.
https://www.nelabservices.com/pdf/tech-sheets/anaerobic-agars-2016.pdf
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