I would like to do a transformation with E. coli ATCC 25922. Would like to simply add a plasmid for amp. resistance. I need to use ATCC 25922 for my research question. Does anyone have a protocol for making these cells competent for transformation?
You can use a standard calcium chloride treatment to make them competent as indicated in
https://openwetware.org/wiki/Gill:Calcium_chloride_competent_cells
a faster but less efficient protocol can be found here:
https://openwetware.org/wiki/Preparing_chemically_competent_cells
usually the concern with working with different ecoli backgrounds rather than the standard lab strains is the difference in restriction-modification system (although we routinely transform plasmids from k12 derivative backgrounds into B background at the cost in the reduction in efficiency), however, this particular strain has been transformed before and the authors did not indicate the need for any special treatment:
Article Chemogenomic Screen for Imipenem Resistance in Gram-Negative Bacteria
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