Sample dilution is done on some complex matrice prior to chromatographic analysis whilst other complex samples are analyzed directly with no sample dilution. what is the significance of sample dilution when using chromatographic techniques?
The dilution will decrease composition of the matrice and the analyte will detector well than. The dilution can determine the matrice effect to method.
Hi, First let clear which chromatographic analysis you need? is it GC or HPLC. Secondary, can you provide more details like diluent or mobile phase (if HPLC).
Achieving higher specificity and selectivity in analytical determinations.
It molecular array of the analyte sprays well.
Hi Samuel Mgiba ,
Basically, two reasons for dilution of samples before analysis:
1) to avoid saturation of detector and remain in the range of the calibration curve;
2) to reduce the matrix effect.
Pranav Pandya I'm planning to use a GCxGC-ToFMS. In relation to the diluent, I'm planning to use toluene and DCM (dichloromethane)
Oda Mizil Roberto Molteni Joseph Aondoaver Gbertyo Can you please refer me to at least one article title where I can read extensively about sample dilution and direct analysis
Sample dilution and in general dilution procedures are part of the usual analytical chemistry courses.
Sample dilution helps in protecting the column and the detector.
To plot the standard curve you need serial dilution of a standard, sample dilution prior to analysis helps in selectivity in analytical determinations of concentration.
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