Hyde Chen

11 Questions 41 Answers 0 Followers

Questions related from Hyde Chen

Does DNA contamination affect RNA quality determination using RIN?

In the FFPE samples, both DNA and RNA are fragmented. Someone does not use DNase I during RNA isolation of the FFPE sample. They found the DV200 was confusing. Does DNA contamination affect RNA...

18 May 2023 7,561 2 View

What is the mechanism of RNA fragmentation?

https://www.thermofisher.com/order/catalog/product/AM8740?SID=srch-hj-AM8740#/AM8740?SID=srch-hj-AM8740 RNA Fragmentation Reagents The average size of the resulting fragments will be 60-200...

13 November 2020 4,313 2 View

Flow cytometry of NSG mice?

I got some NSG-like mice from company(in China). Those mice can survival in lab gangway（not clear） for almost 2 weeks. Does it normal? I want to check their blood through FCM. And I want to use...

28 September 2016 3,048 3 View

Python for primer design and test?

I designed many primers and they will work as a mixture. I am not sure whether those primers bind with each other (instead of template). I try to find some soft to check the quality of those...

17 July 2016 8,189 3 View

Human CFC-Assay: are they clones?(what are they)?

I use the 4034 from stemcell. Seed cell are cd34+ from hES.

01 July 2016 6,999 3 View

How to made immortal leukemia cell?

A friend has a patient with a new mutation (leukemia). He want to keep this mutation to further study. So he collected monocyte from patient and want to make a immortal cell line. He do not know...

27 June 2016 7,591 4 View

What is the relationship between CD25 and CD69 in T cell activation?

T cells express CD25 and CD69 when they are activated. They are synchronous or separated？IL2 can induce CD25 expression. CD69 are induced by what, IL2 or IFN-g?

20 December 2015 5,709 6 View

What is the formula of StemSpan™ SFEM II?

I want to optimize my IMDM for HSC culture via the reference of SFEM II. As you know SFEM II is too expensive. I can only find information as follow: Bovine Serum Albumin,Insulin, Human,...

17 November 2015 1,435 3 View

Is it possible to use pluripotent gene promoter to drive lentiviral vectors?

I want to do hESC differentiation with some TFs, which work at different stages. The ef1a promoter drive genes without control. Dox-TRE system can only control one stage? How to control different...

29 March 2015 429 5 View

Why does the mouse change its coat color after Bone marrow transplantation?

I do the mice BMT from August. The control mice died after 2 week. And the BMT mice were survived after one month. Then I kill the mice to check the GFP(I use the C57 GFP transplant to C57...

19 November 2014 1,274 9 View

Is Single-Cell Sequencing of Bacteria Really Necessary?

Now several scRNA-seq platforms are used in Bacteria. I can not get the point. Firstly, Bacteria have Horizontal inheritance, meaning they can exchange the genome. How to get the ref genomes in...

01 January 1970 614 4 View