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Questions related from Hyde Chen
What happens in snapTotal- seq? (Single-cell Total-RNA Profiling Unveils Regulatory Hubs of Transcription Factors https://www.nature.com/articles/s41467-024-50291-3) In this protocol, several RT...
19 July 2024 10,292 0 View
In the FFPE samples, both DNA and RNA are fragmented. Someone does not use DNase I during RNA isolation of the FFPE sample. They found the DV200 was confusing. Does DNA contamination affect RNA...
18 May 2023 7,669 2 View
Several lysis buffers can isolate nuclei from fresh or frozen tissues. However, I am not sure the ratio of lysis buffer to tissue. Some tissues with muscle and smooth muscle have more debris after...
20 November 2020 4,889 1 View
https://www.thermofisher.com/order/catalog/product/AM8740?SID=srch-hj-AM8740#/AM8740?SID=srch-hj-AM8740 RNA Fragmentation Reagents The average size of the resulting fragments will be 60-200...
13 November 2020 4,455 2 View
Any cell line for motile cilia study in vitro? Or Which kind of cell is typical motile cilia, from lung, testis, or other tissues? how to isolate motile cilia from mice?
08 March 2019 2,698 0 View
In the summer I suffer the mosquito bites. When the bites are on the face, they are fade away next day. However the bites on the leg or waist, they can last several days. (I try not to scratch,...
24 June 2018 6,715 8 View
I used lentivirus before but I know few about the structure of them. Here is an example: ^^^^^^5'LTR-GENE-WPRE-LTR3'-SV40^^^^^ Are only sequences between the LTRs integrated into the genome? Is...
02 June 2017 9,173 4 View
After injecting human HSC into mice, some cells are double positive (human and mice marker). What happened to those cells?
19 January 2017 3,269 4 View
Many small molecules are used in cell culture. How to get their concentration?Small molecules and Paper are too much……almost kill me. Is there a database?
29 October 2016 3,151 4 View
I got some NSG-like mice from company(in China). Those mice can survival in lab gangway（not clear） for almost 2 weeks. Does it normal? I want to check their blood through FCM. And I want to use...
28 September 2016 3,122 3 View
I designed many primers and they will work as a mixture. I am not sure whether those primers bind with each other (instead of template). I try to find some soft to check the quality of those...
17 July 2016 8,254 3 View
I use the 4034 from stemcell. Seed cell are cd34+ from hES.
01 July 2016 7,060 3 View
A friend has a patient with a new mutation (leukemia). He want to keep this mutation to further study. So he collected monocyte from patient and want to make a immortal cell line. He do not know...
27 June 2016 7,664 4 View
Now i can do HSC injection through tibia. But papers always described "injected into the right femur (RF)" . Does this means femur is better? I try do femur injection. However,I can not fix it...
10 May 2016 8,171 2 View
HES on feeder free culture condition. [ matrigel(not of stem cell) ＋ mTeSRTM1 medium (Stemcell Technologies)+ some LV infection (different genes all have same morphological change, so it is not...
09 January 2016 4,211 6 View
T cells express CD25 and CD69 when they are activated. They are synchronous or separated？IL2 can induce CD25 expression. CD69 are induced by what, IL2 or IFN-g?
20 December 2015 5,792 6 View
I want to optimize my IMDM for HSC culture via the reference of SFEM II. As you know SFEM II is too expensive. I can only find information as follow: Bovine Serum Albumin,Insulin, Human,...
17 November 2015 1,504 3 View
I want to do hESC differentiation with some TFs, which work at different stages. The ef1a promoter drive genes without control. Dox-TRE system can only control one stage? How to control different...
29 March 2015 488 5 View
"Furthermore, although KDR/flk-1 (VEGFR2) is a cell surface marker of populations containing murine hemangioblasts, it appears to be less specific for specifying rare hemangioblasts in human...
29 January 2015 5,569 3 View
I read paper "Wnt signaling controls the specification of definitive and primitive hematopoiesis from human pluripotent stem cells"----I confused that why primitive do not have T lymphoid...
25 January 2015 3,712 7 View
Why the HSC derived from HES cannot perform good in transplant(secondly)? What is the marker for long term HSC(not CD34 CD45 ?)?
24 January 2015 8,028 0 View
Using the irradiation to treat BMT (bone marrow transplantation) patients what will happen to the APCs or the immune cells reside in the tissues (DC in the thymus or ^^)? Will the new bone marrow...
26 November 2014 4,180 1 View
I do the mice BMT from August. The control mice died after 2 week. And the BMT mice were survived after one month. Then I kill the mice to check the GFP(I use the C57 GFP transplant to C57...
19 November 2014 1,345 9 View
Now several scRNA-seq platforms are used in Bacteria. I can not get the point. Firstly, Bacteria have Horizontal inheritance, meaning they can exchange the genome. How to get the ref genomes in...
01 January 1970 684 4 View
