29 March 2015 5 439 Report

I want to do hESC differentiation with some TFs, which work at different stages. The ef1a promoter drive genes  without control. Dox-TRE system can only control one stage? How to control different TF expression in several stages? For example, stage I express A and B, stage II express C and D, stage III express E and F. As I know oct4 promoter is huge, does it ok  for Lentiviral Vectors?

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