I do the mice BMT from August. The control mice died after 2 week. And the BMT mice were survived after one month. Then I kill the mice to check the GFP(I use the C57 GFP transplant to C57 NO-GFP ) cell rate in the bone marrow and spleen. Still one mouse is survived and I want to know what will happen.
I found the mouse is aged(appear white color )? and sensitive?
I do not have the SPF environment and I do my best to care them in the first month with clear low pH water and food.
I don't know exactly how much radiation given was (clearly it was lethal but I don't know how much beyond) but it seems that it was enough to damage the hair follicles and most specifically the cells producing the pigments. Highly active cells (such as those producing immune cells and to a lesser extent others such as those that produce hair and it's pigments) are the most sensitive to radiation damage. The most obvious example of this is hair loss in human individuals undergoing radiation treatment as part of a regimen to combat (cure) cancer.
Yep looks like radiation damage (especially photo 2) . . . . I would check the dosage of radiation, you may not need this much to do a bone marrow transplant and the additional damage may influence your results (it's been a while and those experiments were in rats as well so I have no idea at this point).
As has been said, it is due to the radiation given. Have seen this myself in similar experiments.
Speculatively, I would say that radiation exacerbates ageing. Grey or white hair is something naturally occurring in ageing animals (as well as in humans obviously). There are some research I read a couple of years ago where the authors suggest that irradiated bone marrow cells has similarities to bone marrow cells in aged mice that has not received a radiation dose.
This is a radiation induced effect. Actually skin color changes also occur during human transplantation; mainly dark coloring of the skin but this is due to Busulfan used in the conditioning regimen. Also, if skin GVHD occurs; this may cause some changes in the skin color and also in the hair.
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