HES on feeder free culture condition. [ matrigel(not of stem cell) ＋ mTeSRTM1 medium (Stemcell Technologies)+ some LV infection (different genes all have same morphological change, so it is not the problem of genes )]
what is wrong?
Dear Collega;
I cannot explain this change, but it seems to be interesting to investigate its consequences. This may be a proof of the influence of the culture medium on the cell development and a possible way to manage the stem cell differentiation. When you will know where it leads, it may be easier to understand what has happened.
Good luck,
V.Coulic
Dear Hyde,
It looks like in images one and two you have some or a few OK looking cells, but plenty of dead ones and debris (as evident from the granular-vesicular look of the tissue). In the last image cells are total wiped-out, cells all dead and washed out to the medium.
best wishes,
Refik
Cells look spiky it's not uncommon for ES after LV infection but on the pictures it's very accentuated suggesting the possibility of abnormal caryotype, third pic the phase contrast set up is wrong.
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