What is the mechanism of RNA fragmentation?

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How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

How can I find writers for drafting, submitting, and publishing papers?

Looking for paper collaboration writers： Collaboration Model 1: 1.1 Based on the chosen topic, complete the paper writing, select an SCI or SSCI Q1 journal, submit using my QRCID, and complete...

27 July 2024 8,965 1 View

Mechanochemistry: ball to powder mass or ball to powder volume?

I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...

23 July 2024 9,938 1 View

How can I determine if a drug binds to a ribosome using a Cryo-EM map?

Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...

19 July 2024 2,155 0 View

Can anyone recommend a brand of cas9 protein that can be used for in-vivo editing in model animals?

zebrafish and mouse

19 July 2024 9,158 1 View

What happens in snapTotal- seq?

What happens in snapTotal- seq? (Single-cell Total-RNA Profiling Unveils Regulatory Hubs of Transcription Factors https://www.nature.com/articles/s41467-024-50291-3) In this protocol, several RT...

18 July 2024 10,010 0 View

How to cacualte the creep life of polycrystal with CPFEM?

I saw a paper(PHAN V-T, ZHANG X, LI Y, et al. Microscale modeling of creep deformation and rupture in Nickel-based superalloy IN 617 at high temperature [J]. Mechanics of Materials, 2017, 114:...

16 July 2024 8,436 0 View

Does HEK293T cell express RIG1?

Is there any benefit in knocking out the interferon receptor in 293T cells? In the context of creating RNA viruses 📷

13 July 2024 3,382 0 View

The HCP removal rate is lower than usual in concentration step, why?

Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...

11 July 2024 5,380 0 View

The importance of humanistic care?

Humanistic care is mainly about caring for people's living conditions, affirming people's living conditions and dignity, and pursuing human freedom and liberation. With the continuous development...

10 July 2024 5,107 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Is it true that the vacuum bomb is about to arrive?

We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...

04 August 2024 4,534 1 View