T cells express CD25 and CD69 when they are activated. They are synchronous or separated？IL2 can induce CD25 expression. CD69 are induced by what, IL2 or IFN-g?
Both are up regulated upon Tc activation via TCR signaling but with different kinetics. CD69 is a very early activation marker and is detectable within hours of TCR ligation then expression is lost after 48-72 hours. CD25 is the alpha chain of the IL-2 receptor and is up regulated a bit later than CD69- it is detectable on day 1 and up regulated ~day 2 following TCR ligation. CD25 expression remains high out to day 4-5 then starts to go down on day 7. By day 11 it is back to baseline.
CD69 expression is dependent on activation via TCR. Its expression is induced with anti-CD3 + IL-2
As Sandra has mentioned, CD69 is regarded as an early activation marker. It may be upregulated within a few hours. However, its downregulation appears to be dependent on the assay/enviroment. Whereas I had in the past observed diminished CD69 expression a few days after activation, some colleagues of mine use CD69 to detect activated cells under chronic conditions.
CD69 is induced by T-cells right after the TCR down-regulation whereas CD25 takes comparatively more time. In terms of their functions, CD25 helps to understand the behavior of other T-cells whereas CD69 give strength to the activation process which actually helps T-cells to induce more CD25. In terms of their variation on the cell surface, CD69 and CD25 show very similar behavior and one can say that CD69 is just a bit more active than CD25. For example, CD69 appears early on the surface than CD25 and disappears early as well and CD69 concentration is observed higher than CD25.
What happen to t-cells in chronic diseases? do they continue to express CD25 or do they lose both markers?
To Markus Biburger: how do your colleagues use CD69 under chronic conditions, if it's expressed in the early phase of the activation?
I am trying to look for CD44, CD25 and Glut1 after 24hrs activation with Anti-CD3/CD28 dynabeads using human PBMCs. But am not seeing signal for Glut1 and CD25 except CD44. Can anyone help with suggestion why this happen or how to improve
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