37 Questions 62 Answers 0 Followers
Questions related from Hanie Kavand
I use the typical syringe pumps to load organoids (200-300 um in diameter) into my device; however, I don't see them in the chip! I swapped them with beads (similar size) instead and observed that...
27 February 2023 991 2 View
I am trying to immunostain whole (micro)tissue samples, and I think there is an antibody penetration issue. Would longer permeabilization procedures or harsher permeabilization agents help with...
15 February 2023 5,931 4 View
It's quite confusing to distinguish between the definitions. To me, autofluorescence fits all. But what is the correct terminology to use when talking about biological cells, proteins (such as...
15 February 2023 1,015 5 View
I was wondering if it is possible to add a second photoinitiator to SU-8 to photopolymerize it at higher wavelengths. Theoretically, it seems doable, but I'm unsure about the chemistry~
17 January 2023 1,699 2 View
I assume that the antibody recognizes and attaches to the protein from the outer cell membrane. So, maybe there's no need for permeabilization? Bonus question: can we run sequential staining for...
25 November 2022 8,546 2 View
I am trying to characterize the foreign body reaction surrounding my implant, and all the cells are stained positive for CD31. I am not sure if they should be! (I am implanting my material in the...
23 November 2022 8,655 4 View
We are currently using Ketamine and Xylazine to anesthetize the mice for imaging purposes. However, upon injection, we see an increase in the blood glucose levels, which in turn, prevents us from...
20 October 2022 1,955 5 View
I am using a sterilized GelMA to print structures using two-photon polymerization techniques. The printing procedure is carried out outside a sterilized (laminar flow hood, for example)...
18 October 2022 3,836 2 View
I am interested in seeing the 3D image of the anterior chamber of the mouse eye. Due to the complexity of ethical permits, transporting the mice to the OCT scanner is challenging and will take a...
18 October 2022 5,137 3 View
I am interested in showing innervation in the anterior chamber of the eye, in live mice. Is there any dye I could use?
02 October 2022 1,656 1 View
I am wondering if a (semi) solid substrate/scaffold (could be a hydrogel) is necessary for endothelial cells to sprout out to a VEGF producing source. In other words, it it possible to have...
08 August 2022 3,285 2 View
I am assuming that endocrine cells should have no to minimum distance to the blood stream, so that the secreted hormone could be directly secreted to the blood, without passing through the...
20 July 2022 2,163 2 View
I would like to know if it is possible to use the the calcium indicator dyes to stain tissues in a live animal. I am not talking about genetically encoded calcium indicators of course. My main...
17 July 2022 9,710 6 View
I am transplanting a polymer (Parylene-C) coated structure in the anterior chamber of the eye and expect it to reside on the iris, however, almost all of them get adhered to the cornea and not the...
21 January 2022 7,195 3 View
By a 'higher differentiation capacity' I mean that it would be easier to differentiate them– especially without adding a pool of chemical factors. I think cell lines might be tricky as they are...
21 January 2022 5,403 5 View
I have always etched Si to create masters for PDMS molding and the etch depth never went beyond 200 um. Now, I need to create structures with a high of 450-500 um and the smallest feature size is...
27 October 2021 1,200 6 View
I am trying to coat SU-8 on a photodiode (400x400 um) via spray coating. The coating isn't uniform and the SU-8 doesn't seem to cover all the surface; it looks like it is affected by the surface...
18 October 2021 7,753 5 View
I am using a neutralized collagen and upon cross linkage in the incubator, the gel becomes opaque. Is there a way to make it perfectly transparent?
18 March 2021 8,957 0 View
I am looking for ways to give orientation to micro-scaled silicon chips and I am thinking of using a 'life jacket' strategy to keep one side always floating and facing upwards. Are there any...
23 February 2021 7,027 10 View
For characterizing a photodiode, I will be using an epifluorescence microscope as the illumination source. While running the measurements, there is a lot of noise that makes real signal (in nAmps...
17 February 2021 4,599 8 View
I am trying to quantify Parylene C's hermetic encapsulation quality using EIS with a 2 electrode configuration (WE/WS and RE/CE). I am simply using two patterned gold layers on glass as my...
13 January 2021 7,713 14 View
I need perfect cleanliness for further processes but I am not sure which cleaning method would be sufficient yet letting the layers intact.
02 December 2020 1,342 2 View
How can we quantify how many active GECIs are present within a cell and how can we calculate their lifetime? Also, as I guess, we can't play with the fluorescent prob concentration in GECIs...
16 June 2020 2,602 0 View
A simple definition might help to understand the diffraction pattern in an aperiodic nano-micro structured surface.
20 July 2018 4,139 6 View
I am using glutaraldehyde to fix cells on glass slides. However, in the next process I want to detach these cells off the slide. Is there any detaching process defined for such situations? An...
06 October 2016 6,755 1 View
I need to label my cells with a nano particle and I don't want the particles to enter cells for a time period of 21 days.
02 June 2016 5,329 3 View
RGD specifically binds to the integrin receptors, which makes helps with the nanoparticle-cell conjugate. Even though I am not using any internalisation technique (like electroporation or etc.), I...
25 December 2015 6,497 4 View
What is the most simple way of doing so and how strong is the bondings?
18 December 2015 3,741 1 View
Breast cells are epithelial in origin, however during EMT, the epithelial phenotype (and maybe genotype) is replaced by by the mesenchymal phenotype. This makes me wonder if we have a mesenchymal...
25 November 2015 8,862 6 View
Why do researchers apply different cancer cell lines for their research purposes? I know that the cell lines differ in "invasiveness" potentials, but what does this mean? for example what is the...
25 November 2015 8,689 5 View
For example what proteins and receptors are produced in normal breast cells and malignant cells.
25 November 2015 7,684 3 View
Can tuning roughness leads to different stiffness of a material?
19 October 2015 3,479 8 View
Weird or not, I've come across a range starting from 30 Pa to 5 GPa! Can someone please explain this range?
06 October 2015 9,309 2 View
I am looking for the gene expression profile for lung cancer, however the articles I come across are so confusing and discuss a large range of genes that leave me hanging in limbo! That's why I am...
22 September 2015 5,523 6 View
As trypan blue fails to calculate the accurate viability rate of my [cell+alginate 1.2%] suspension, I was thinking about using dual florescent dye (PI & AO). However, I am concerned about whether...
23 February 2013 5,319 2 View
While encapsulating ADMSCs in sodium alginate, providing a 3D environment for the cells, I come across different viability rates. Trypan blue shows a lower viability rate, as low as 50%. On the...
20 February 2013 6,397 5 View
As soon as I add alginate to the pelleted cells, no matter what type of cell it is i.e. fibroblast or ADSC, the 100% viability decreases to 50%. I have even tried [alginate + cell culture medium],...
18 February 2013 3,688 14 View
